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- Tue Feb 06, 2018 1:47 pm
- Forum: General
- Topic: Best instrument for heat retreaval in FFPE
- Replies: 8
- Views: 4888
We have spent a lot of time testing different antigen retrieval methods, including proteases, pH, pressure and subsequently >100 degree temperatures, and microwaves. Although some of the more extreme conditions can make a difference, they are often not reproducible. We have had reproducibility issue...
While we do not have experience with the Ventana stainer for IMC or heavy metal conjugated antibodies, we did some work a few years ago with a Ventana stainer and Cy5 tyramide. We found that the standard Cy5 tyramide was incompatible with the liquid coverslip, possibly partitioning in the oil phase ...