Search found 2 matches

by drimm
Tue Feb 06, 2018 1:47 pm
Forum: General
Topic: Best instrument for heat retreaval in FFPE
Replies: 8
Views: 4888

Re: Best instrument for heat retreaval in FFPE

We have spent a lot of time testing different antigen retrieval methods, including proteases, pH, pressure and subsequently >100 degree temperatures, and microwaves. Although some of the more extreme conditions can make a difference, they are often not reproducible. We have had reproducibility issue...
by drimm
Sat Jan 20, 2018 8:11 am
Forum: Reagents
Topic: Ventana Staining
Replies: 3
Views: 2499

Re: Ventana Staining

While we do not have experience with the Ventana stainer for IMC or heavy metal conjugated antibodies, we did some work a few years ago with a Ventana stainer and Cy5 tyramide. We found that the standard Cy5 tyramide was incompatible with the liquid coverslip, possibly partitioning in the oil phase ...