Search found 3 matches

by mleipold
Thu Jun 18, 2020 7:39 pm
Forum: General
Topic: Survey on dataset sharing and reuse
Replies: 0
Views: 262

Survey on dataset sharing and reuse

Hi all, I'm cross-posting this from Cytoforum ( http://cytoforum.stanford.edu/viewtopic.php?f=1&t=1937 ) and the Purdue flow cytometry mailing list, in an effort to get maximum visibility on this effort. ---------------------------- There was a thread about a year ago on dataset sharing and reuse: v...
by mleipold
Thu Nov 08, 2018 7:02 pm
Forum: Publications & Resources
Topic: Nature Methods-call for biological imaging archive database
Replies: 0
Views: 5179

Nature Methods-call for biological imaging archive database

Cross-posting from Cytoforum: "A call for public archives for biological image data" Jan Ellenberg, Jason R. Swedlow, Mary Barlow, Charles E. Cook, Ugis Sarkans, Ardan Patwardhan, Alvis Brazma & Ewan Birney Nature Methods, 2018, 15, 849–854 (2018) https://doi.org/10.1038/s41592-018-0195-8 -Table 1 s...
by mleipold
Wed Feb 28, 2018 6:27 pm
Forum: Reagents
Topic: Antibody clean up prior to conjugation
Replies: 3
Views: 5650

Re: Antibody clean up prior to conjugation

Hi Akil, Gelatin does not have any Cys, therefore it won't interfere in the conjugation reaction (ie, won't react with the MAXPAR maleimide instead of your antibody). The main issue with gelatin is that if it's not removed, it might affect your protein concentration determination (Nanodrop reading) ...