Search found 2 matches

by mleipold
Thu Nov 08, 2018 7:02 pm
Forum: Publications & Resources
Topic: Nature Methods-call for biological imaging archive database
Replies: 0
Views: 1023

Nature Methods-call for biological imaging archive database

Cross-posting from Cytoforum: "A call for public archives for biological image data" Jan Ellenberg, Jason R. Swedlow, Mary Barlow, Charles E. Cook, Ugis Sarkans, Ardan Patwardhan, Alvis Brazma & Ewan Birney Nature Methods, 2018, 15, 849–854 (2018) https://doi.org/10.1038/s41592-018-0195-8 -Table 1 s...
by mleipold
Wed Feb 28, 2018 6:27 pm
Forum: Reagents
Topic: Antibody clean up prior to conjugation
Replies: 3
Views: 1640

Re: Antibody clean up prior to conjugation

Hi Akil, Gelatin does not have any Cys, therefore it won't interfere in the conjugation reaction (ie, won't react with the MAXPAR maleimide instead of your antibody). The main issue with gelatin is that if it's not removed, it might affect your protein concentration determination (Nanodrop reading) ...