Search found 3 matches

by erikg
Wed Mar 14, 2018 3:48 pm
Forum: Data analysis
Topic: Preprocessing and normalization in histoCAT
Replies: 6
Views: 7321

Preprocessing and normalization in histoCAT

What do pre-processing step would you recommend prior to importing the uint16 images into HistoCat (hotspot removal etc)? How do you best normalize your images when combining larger image sets(ROIs) that you want to analyse with tSNE/Phenograph clustering.
by erikg
Fri Feb 09, 2018 3:06 pm
Forum: Data analysis
Topic: Visualization of Compensated Images
Replies: 7
Views: 9179

Re: Visualization of Compensated Images

Thanks Vito!
by erikg
Fri Jan 12, 2018 6:43 pm
Forum: Data analysis
Topic: Visualization of Compensated Images
Replies: 7
Views: 9179

Re: Visualization of Compensated Images

Thanks for clarifying the rational behind the OME TIFF, I am also not a big fan of the mcd format and would alos prefer a different format, the problem is a you also pointed out that you anyway need to convert it again for most other applications. We store the txtfiles as rds in the R pipeline (and ...