Cd conjugates (MCP9) in IMC

Does this antibody work? Designing a new panel?
Antibodies, isotopes, etc. for Imaging Mass Cytometry applications
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DesireeCharite
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Joined: Thu Nov 01, 2018 7:12 pm

Cd conjugates (MCP9) in IMC

Post by DesireeCharite » Wed May 13, 2020 1:34 pm

Hi,
I know that Fluidigm's advise is not use the MCP9 Cd-conjugates in IMC, but of course I am curious…
Apparently they have seen unspecific binding of these antibodies. Has anyone given it a try? Do you know if the unspecific background staining is the only thing arguing against it or are there other more harmful effects to the instrument, like residues sticking to parts in the instrument?

Kind regards
Desiree
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