Search found 9 matches

by mattbliss90
Mon Mar 04, 2019 9:16 pm
Forum: Reagents
Topic: H&E and IMC staining
Replies: 1
Views: 92

H&E and IMC staining

Hello,
Has anyone tried staining the same FFPE slide with haematoxylin and eosin and then an IMC antibody cocktail? If so, does the H&E stain have any negative effect on the IMC antibody binding and the expected end results after running through the Hyperion?

Matt
by mattbliss90
Tue Nov 13, 2018 7:00 pm
Forum: Reagents
Topic: Inter cellular stain on mouse frozen tissue
Replies: 1
Views: 896

Inter cellular stain on mouse frozen tissue

Hello, Has anyone here ever tried doing an inter-cellular stain on frozen mouse tissue? If so what protocol do you use... do you do a methanol permeabilization before adding all the surface antibodies and IC antibodies, or split the surface antibody incubation and IC antibody incubation into two dif...
by mattbliss90
Mon Aug 20, 2018 11:36 pm
Forum: Reagents
Topic: Mouse FFPE antibodies
Replies: 5
Views: 1017

Re: Mouse FFPE antibodies

Hart and Santhosh,
The clones I listed above have been previously validated and are routinely used in the histology core facility at my company for IHC on mouse FFPE sections.

Matt
by mattbliss90
Fri Aug 17, 2018 6:26 pm
Forum: Reagents
Topic: Mouse FFPE antibodies
Replies: 5
Views: 1017

Mouse FFPE antibodies

Hello, I am trying to establish a basic antibody panel to be used in mouse FFPE spleen but have seen very poor results. Has anybody had any success conjugating their own antibodies from established clones in-house and using them on mouse FFPE sections? So far some of the antibody clones we have trie...
by mattbliss90
Thu Jul 26, 2018 8:12 pm
Forum: Data analysis
Topic: Cell population frequencies in HistoCat
Replies: 10
Views: 1740

Re: Cell population frequencies in HistoCat

Hello Denis,
We would be happy to test out the pre-release HistoCat 1.8

Thanks,
Matt
by mattbliss90
Tue Jul 24, 2018 6:12 pm
Forum: Data analysis
Topic: Cell population frequencies in HistoCat
Replies: 10
Views: 1740

Cell population frequencies in HistoCat

Hello,
Is it possible to obtain cell population frequencies/numbers directly in the HistoCat software without having to export the FCS or CSV file into another software such as FloJo?

Matt
by mattbliss90
Fri Jul 06, 2018 7:02 pm
Forum: Reagents
Topic: Counterstain using RuO4
Replies: 4
Views: 887

Re: Counterstain using RuO4

Monirath,
We tried it a few weeks back after the DNA intercalator stain. It worked well, but it is fairly strong so I wouldn't stain longer than outlined in the paper.

Matt
by mattbliss90
Fri Jul 06, 2018 6:52 pm
Forum: Data analysis
Topic: Help with neighborhood analysis
Replies: 8
Views: 1220

Help with neighborhood analysis

Hello, I am having trouble getting the neighborhood analysis to work on Histocat. As outlined in the manual I select the ROI in Samples/Populations and then click "neighborhood analysis" next to the prepare button and fill in the cluster number on the new pop up window and then select the phenograph...
by mattbliss90
Wed Jan 17, 2018 11:44 pm
Forum: Reagents
Topic: Long term storage IMC antibodies
Replies: 3
Views: 1182

Long term storage IMC antibodies

Does anyone have any suggestions for a buffer for long term storage of hand conjugated IMC antibodies? I know Fluidigm recommends Boca Scientific who has antibody stabilization buffers, however that vendor is not in my companies approved vendor list and I'm looking for alternatives.