Search found 8 matches
- Wed Mar 28, 2018 8:29 pm
- Forum: Publications & Resources
- Topic: IMC compensation paper
- Replies: 0
- Views: 7514
- Tue Mar 13, 2018 2:50 pm
- Forum: Reagents
- Topic: Long term storage IMC antibodies
- Replies: 3
- Views: 5952
Re: Long term storage IMC antibodies
Dear monirathhav, This topic has been extensively discussed previously, please find a link here http://cytoforum.stanford.edu/viewtopic.php?f=4&t=633&p=1963&hilit=antibody+storage#p1963 Many antibodies will still work well after 1 year, but some do not. We never really figured out what makes the dif...
- Tue Mar 13, 2018 2:48 pm
- Forum: Reagents
- Topic: Antibody clean up prior to conjugation
- Replies: 3
- Views: 8375
Re: Antibody clean up prior to conjugation
Hi Akil, My co-workers Andrea Jacobs and Stefanie Engler once looked into this. They found that for suspension MC up to 0.1 % BSA (depending on the antibody concentration) is tolerated. They also tested the same antibodies for IMC and found that these conjugates produce a higher background staining ...
- Fri Jan 19, 2018 8:57 am
- Forum: Reagents
- Topic: Ventana Staining
- Replies: 3
- Views: 5984
Re: Ventana Staining
We do not have experience with the Ventana machines, but Hartland Jackson in my lab compared O/N@4°C with 1h@RT antibody staining and he got much more background with the 1h@RT antibody staining. Thus we prefer the O/N@4°C condition. But who knows, things might be different with the Ventana set-up a...
- Thu Jan 18, 2018 10:47 pm
- Forum: Careers
- Topic: Postdoc positions
- Replies: 0
- Views: 8230
Postdoc positions
We are looking for a postdoc studying fibroblasts in lung cancer using Imaging Mass Cytometry.
https://www.nature.com/naturejobs/scien ... -of-zurich
https://www.nature.com/naturejobs/scien ... -of-zurich
- Thu Jan 18, 2018 10:38 pm
- Forum: Reagents
- Topic: Long term storage IMC antibodies
- Replies: 3
- Views: 5952
Re: Long term storage IMC antibodies
We are using since many years an antibody stabilizer from Candor (https://www.candor-bioscience.de/en/pro ... lizer.html) for antibody storage at 4C.
Re: Welcome
Thanks! I am looking forward to the questions and comments from Houston.
Welcome
Welcome to the new Imaging Mass Cytometry User Forum! This forum was inspired by the exciting discussions we had during our first imaging mass cytometry summit and workshop in Zurich in 2017. I am truly excited about the possibilities imaging mass cytometry provides and confident that we will be abl...