Dear monirathhav, This topic has been extensively discussed previously, please find a link here http://cytoforum.stanford.edu/viewtopic.php?f=4&t=633&p=1963&hilit=antibody+storage#p1963 Many antibodies will still work well after 1 year, but some do not. We never really figured out what makes the dif...

Hi Akil, My co-workers Andrea Jacobs and Stefanie Engler once looked into this. They found that for suspension MC up to 0.1 % BSA (depending on the antibody concentration) is tolerated. They also tested the same antibodies for IMC and found that these conjugates produce a higher background staining ...

We do not have experience with the Ventana machines, but Hartland Jackson in my lab compared O/N@4°C with 1h@RT antibody staining and he got much more background with the 1h@RT antibody staining. Thus we prefer the O/N@4°C condition. But who knows, things might be different with the Ventana set-up a...

We are looking for a postdoc studying fibroblasts in lung cancer using Imaging Mass Cytometry.
https://www.nature.com/naturejobs/scien ... -of-zurich

We are using since many years an antibody stabilizer from Candor (https://www.candor-bioscience.de/en/pro ... lizer.html) for antibody storage at 4C.

Thanks! I am looking forward to the questions and comments from Houston.

Welcome to the new Imaging Mass Cytometry User Forum! This forum was inspired by the exciting discussions we had during our first imaging mass cytometry summit and workshop in Zurich in 2017. I am truly excited about the possibilities imaging mass cytometry provides and confident that we will be abl...