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Counterstain on mouse tissue

Posted: Mon May 06, 2019 9:21 am
by kuehla
What do you use for counterstain on mouse tissue selctions (FFPE or cryo)?
In our hands, Ir 191/193 works not as good as on human tissue sections, but the signal is okayish. Using Histone H3 gives a cytoplasmic signal... Should we try RuO4?

Re: Counterstain on mouse tissue

Posted: Fri Aug 02, 2019 8:10 am
by RaulCatena
I highly recommend RuO4,
It is easy and fast and we know that people from other labs have adopted it without problems.

Best regards,