Standardized retrieval conditions?
Posted: Mon Feb 04, 2019 2:52 pm
Hi all,
As you all know, retrieval is key to staining on FFPE tissues, especially when trying to get 35 antibodies to work well together.
What retrieval conditions have you been having most success with for the broadest range of antibodies and tissues? (Citr/EDTA, temp, time)
I have tried multiple ones including 95C/10 min, 20 min, 30 min; 121C/5 min in pressure cooker. High temp seems to work well for most but some antibodies are still problematic.
It would be very helpful to hear everyone's experience so far.
Thanks,
Santhosh
As you all know, retrieval is key to staining on FFPE tissues, especially when trying to get 35 antibodies to work well together.
What retrieval conditions have you been having most success with for the broadest range of antibodies and tissues? (Citr/EDTA, temp, time)
I have tried multiple ones including 95C/10 min, 20 min, 30 min; 121C/5 min in pressure cooker. High temp seems to work well for most but some antibodies are still problematic.
It would be very helpful to hear everyone's experience so far.
Thanks,
Santhosh