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PD-1 antibody

Posted: Wed Dec 11, 2019 9:38 pm
by ssivajothi
Hi all,

Does anyone know of a clone of PD-1 that works well for acetone fixed frozen sections (human)?

Thank you!
Santhosh

Re: PD-1 antibody

Posted: Mon Mar 09, 2020 3:10 pm
by eliom
Hi ssivajothi,

I'm also looking for PD-1 antibody to use on FFPE sections from human duodenum. I tried the one from Fluidigm, but I get a lot of background. Did you find something else that seems to work on human FFPE sections?

Many thanks!
Elio

Re: PD-1 antibody

Posted: Sun May 10, 2020 2:13 pm
by HenrikeSal
Hi Elio and Santhosh,

I've had problems with the PD-1 antibody from fluidigm on human FFPE liver sections as well. I have now tried the D4W2J clone from cell signaling and the NAT105 clone from BioLegend which both work better. The signal intensities are still quite low but at least seem to be specific. I hope this helps.
I haven't tried anything on frozen sections though, sorry.

Best,
Henrike