Issue with running Phenograph

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Computational image analysis of multiplexed Imaging Mass Cytometry acquisitions
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13sjc10
Posts: 1
Joined: Sun Sep 13, 2020 10:06 pm

Issue with running Phenograph

Post by 13sjc10 » Sun Sep 13, 2020 10:15 pm

Hi all,

I'm trying to run Phenograph on my samples and keep running into an issue where some of the generated clusters are comprised of random channels that weren't included in my original selection of channels. I've also been running into a dialogue box that asks about a random seed when I Run Phenograph (picture attached) and am wondering if this might have anything to do with it? Does anyone know what it means? Thank you!
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