Can we reduce Ab noise in downstream analysis?

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Computational image analysis of multiplexed Imaging Mass Cytometry acquisitions
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Hinsamoy
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Joined: Sun Sep 20, 2020 2:33 am

Can we reduce Ab noise in downstream analysis?

Post by Hinsamoy » Sun Sep 20, 2020 2:43 am

Hi all:
Our group is new to the IMC field. We are using clinical FFPE samples for 30 Abs staining. Problem is that we don't have enough samples for testing the best Ab usage in the IMC staining (BTW our panel contains Fluidigm Ab and also labeled Ab by ourselves).
I am trying downstream analysis on our testing data.
I want to know is there any step can lower the Ab-staining noise to get a better clustering and neighbor analysis result.
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