Detecting mouse monocytes on FFPE?

Does this antibody work? Designing a new panel?
Antibodies, isotopes, etc. for Imaging Mass Cytometry applications
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douglas.linn
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Joined: Mon Jan 22, 2018 4:20 pm

Detecting mouse monocytes on FFPE?

Post by douglas.linn » Tue Jan 29, 2019 9:04 pm

Hi All,
Has anyone has success detecting mouse monocytes using mouse FFPE tissues? Using IHC, I can readily use Ly6C (ER-MP20) or CD115 (sigma polyclonal), but neither of these antibodies adapt well to IMC. Does anyone have any other clones that work in their hands? Thanks!
-Doug

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